AFN-000520-01 · EGFR L858R/T790M
RejectedCRV-2026-00403-025 · v1 · auto-fitPlates PL-2026-0403, PL-2026-0404 (n = 2 replicates)4PL · Levenberg–Marquardt · unweighted, log₁₀[C]
Curve 7 / 10 in batch
Response vs concentration
- Rep. 1 · PL-0403
- Rep. 2 · PL-0404
- Masked
- IC50 95 % CI
Concentrations: 10-point, 1:3, 0.508 nM – 10.0 µMPoints used 20 / 20
Fit parameters — v1 · auto-fit
y = Bottom + (Top − Bottom) / (1 + 10^((log IC50 − x) · nH))| Parameter | Estimate | 95 % CI | Constraint |
|---|---|---|---|
| Top | 100.0 % | [97.1, 103.0] % | Free |
| Bottom | 6.6 % | [4.7, 8.4] % | Free |
| IC50 | 193 nM | [171, 219] nM | Free |
| pIC50 | 6.71 | ± 0.03 (SE) | Derived |
| Hill slope (nH) | 0.99 | [0.88, 1.11] | Free |
R² 0.998RMSE 1.7 %df 16Fit engine 1.2.0 · 20 / 20 points
Review decision
IC50
193 nM
95 % CI 171–219 nM · pIC50 6.71
Rejected: Curve does not plateau — retest over a wider range
History
append-only- v1 auto-fit createdsystem · 2026-11-04 17:05:40 UTCFit engine 1.2.0 · 20 / 20 points · no flags
- Rejectedmlaurent · 2026-11-04 18:10:00 UTC“Curve does not plateau — retest over a wider range” · v1 · auto-fit