AFN-001975-01 · EGFR L858R/T790M
RejectedCRV-2026-00415-004 · v1 · auto-fitPlates PL-2026-0415, PL-2026-0416 (n = 2 replicates)4PL · Levenberg–Marquardt · unweighted, log₁₀[C]
Curve 8 / 10 in batch
Response vs concentration
- Rep. 1 · PL-0415
- Rep. 2 · PL-0416
- Masked
- IC50 95 % CI
Concentrations: 10-point, 1:3, 0.508 nM – 10.0 µMPoints used 20 / 20
Fit parameters — v1 · auto-fit
y = Bottom + (Top − Bottom) / (1 + 10^((log IC50 − x) · nH))| Parameter | Estimate | 95 % CI | Constraint |
|---|---|---|---|
| Top | 100.3 % | [99.5, 101.1] % | Free |
| Bottom | 6.7 % | [4.8, 8.7] % | Free |
| IC50 | 10.9 nM | [10.2, 11.7] nM | Free |
| pIC50 | 7.96 | ± 0.01 (SE) | Derived |
| Hill slope (nH) | 1.12 | [1.05, 1.20] | Free |
R² 0.999RMSE 0.9 %df 16Fit engine 1.2.0 · 20 / 20 points
Review decision
IC50
10.9 nM
95 % CI 10.2–11.7 nM · pIC50 7.96
Rejected: Signal drift across the plate, replicates disagree
History
append-only- v1 auto-fit createdsystem · 2026-11-04 17:05:40 UTCFit engine 1.2.0 · 20 / 20 points · no flags
- Rejectedmlaurent · 2026-11-04 18:10:00 UTC“Signal drift across the plate, replicates disagree” · v1 · auto-fit