AFN-003292-01 · EGFR L858R/T790M
RejectedCRV-2026-00428-020 · v1 · auto-fitPlates PL-2026-0428, PL-2026-0429 (n = 2 replicates)4PL · Levenberg–Marquardt · unweighted, log₁₀[C]
Curve 4 / 10 in batch
Response vs concentration
- Rep. 1 · PL-0428
- Rep. 2 · PL-0429
- Masked
- IC50 95 % CI
Concentrations: 10-point, 1:3, 0.508 nM – 10.0 µMPoints used 20 / 20
Fit parameters — v1 · auto-fit
y = Bottom + (Top − Bottom) / (1 + 10^((log IC50 − x) · nH))| Parameter | Estimate | 95 % CI | Constraint |
|---|---|---|---|
| Top | 101.4 % | [99.4, 103.5] % | Free |
| Bottom | 4.8 % | [3.2, 6.3] % | Free |
| IC50 | 144 nM | [132, 157] nM | Free |
| pIC50 | 6.84 | ± 0.02 (SE) | Derived |
| Hill slope (nH) | 1.14 | [1.04, 1.24] | Free |
R² 0.999RMSE 1.5 %df 16Fit engine 1.2.0 · 20 / 20 points
Review decision
IC50
144 nM
95 % CI 132–157 nM · pIC50 6.84
Rejected: Curve does not plateau — retest over a wider range
History
append-only- v1 auto-fit createdsystem · 2026-11-04 17:05:40 UTCFit engine 1.2.0 · 20 / 20 points · no flags
- Rejectedmlaurent · 2026-11-04 18:10:00 UTC“Curve does not plateau — retest over a wider range” · v1 · auto-fit