AFN-003349-01 · EGFR L858R/T790M
RejectedCRV-2026-00430-002 · v1 · auto-fitPlates PL-2026-0430, PL-2026-0431 (n = 2 replicates)4PL · Levenberg–Marquardt · unweighted, log₁₀[C]
Curve 8 / 10 in batch
Response vs concentration
- Rep. 1 · PL-0430
- Rep. 2 · PL-0431
- Masked
- IC50 95 % CI
Concentrations: 10-point, 1:3, 0.508 nM – 10.0 µMPoints used 20 / 20
Fit parameters — v1 · auto-fit
y = Bottom + (Top − Bottom) / (1 + 10^((log IC50 − x) · nH))| Parameter | Estimate | 95 % CI | Constraint |
|---|---|---|---|
| Top | 99.6 % | [95.4, 103.9] % | Free |
| Bottom | −0.5 % | [−1.9, 0.9] % | Free |
| IC50 | 642 nM | [564, 732] nM | Free |
| pIC50 | 6.19 | ± 0.03 (SE) | Derived |
| Hill slope (nH) | 1.03 | [0.92, 1.15] | Free |
R² 0.998RMSE 1.6 %df 16Fit engine 1.2.0 · 20 / 20 points
Review decision
IC50
642 nM
95 % CI 564–732 nM · pIC50 6.19
Rejected: Curve does not plateau — retest over a wider range
History
append-only- v1 auto-fit createdsystem · 2026-11-04 17:05:40 UTCFit engine 1.2.0 · 20 / 20 points · no flags
- Rejectedmlaurent · 2026-11-04 18:10:00 UTC“Curve does not plateau — retest over a wider range” · v1 · auto-fit