AFN-003393-01 · EGFR L858R/T790M
RejectedCRV-2026-00430-006 · v1 · auto-fitPlates PL-2026-0430, PL-2026-0431 (n = 2 replicates)4PL · Levenberg–Marquardt · unweighted, log₁₀[C]
Curve 2 / 10 in batch
Response vs concentration
- Rep. 1 · PL-0430
- Rep. 2 · PL-0431
- Masked
- IC50 95 % CI
Concentrations: 10-point, 1:3, 0.508 nM – 10.0 µMPoints used 20 / 20
Fit parameters — v1 · auto-fit
y = Bottom + (Top − Bottom) / (1 + 10^((log IC50 − x) · nH))| Parameter | Estimate | 95 % CI | Constraint |
|---|---|---|---|
| Top | 100.8 % | [100.0, 101.6] % | Free |
| Bottom | 6.8 % | [5.8, 7.7] % | Free |
| IC50 | 45.8 nM | [43.8, 47.8] nM | Free |
| pIC50 | 7.34 | ± 0.01 (SE) | Derived |
| Hill slope (nH) | 1.17 | [1.11, 1.22] | Free |
R² 1.000RMSE 0.7 %df 16Fit engine 1.2.0 · 20 / 20 points
Review decision
IC50
45.8 nM
95 % CI 43.8–47.8 nM · pIC50 7.34
Rejected: Curve does not plateau — retest over a wider range
History
append-only- v1 auto-fit createdsystem · 2026-11-04 17:05:40 UTCFit engine 1.2.0 · 20 / 20 points · no flags
- Rejectedmlaurent · 2026-11-04 18:10:00 UTC“Curve does not plateau — retest over a wider range” · v1 · auto-fit