AFN-003726-01 · EGFR L858R/T790M
RejectedCRV-2026-00432-026 · v1 · auto-fitPlates PL-2026-0432, PL-2026-0433 (n = 2 replicates)4PL · Levenberg–Marquardt · unweighted, log₁₀[C]
Curve 4 / 10 in batch
Response vs concentration
- Rep. 1 · PL-0432
- Rep. 2 · PL-0433
- Masked
- IC50 95 % CI
Concentrations: 10-point, 1:3, 0.508 nM – 10.0 µMPoints used 20 / 20
Fit parameters — v1 · auto-fit
y = Bottom + (Top − Bottom) / (1 + 10^((log IC50 − x) · nH))| Parameter | Estimate | 95 % CI | Constraint |
|---|---|---|---|
| Top | 101.0 % | [100.4, 101.6] % | Free |
| Bottom | 6.8 % | [4.8, 8.8] % | Free |
| IC50 | 6.12 nM | [5.75, 6.51] nM | Free |
| pIC50 | 8.21 | ± 0.01 (SE) | Derived |
| Hill slope (nH) | 1.09 | [1.03, 1.15] | Free |
R² 1.000RMSE 0.7 %df 16Fit engine 1.2.0 · 20 / 20 points
Review decision
IC50
6.12 nM
95 % CI 5.75–6.51 nM · pIC50 8.21
Rejected: Curve does not plateau — retest over a wider range
History
append-only- v1 auto-fit createdsystem · 2026-11-04 17:05:40 UTCFit engine 1.2.0 · 20 / 20 points · no flags
- Rejectedmlaurent · 2026-11-04 18:10:00 UTC“Curve does not plateau — retest over a wider range” · v1 · auto-fit