AFN-000161-01 · EGFR WT
RejectedCRV-2026-00440-009 · v1 · auto-fitPlates PL-2026-0440, PL-2026-0441 (n = 2 replicates)4PL · Levenberg–Marquardt · unweighted, log₁₀[C]
Curve 9 / 10 in batch
Response vs concentration
- Rep. 1 · PL-0440
- Rep. 2 · PL-0441
- Masked
- IC50 95 % CI
Concentrations: 10-point, 1:3, 0.508 nM – 10.0 µMPoints used 20 / 20
Fit parameters — v1 · auto-fit
y = Bottom + (Top − Bottom) / (1 + 10^((log IC50 − x) · nH))| Parameter | Estimate | 95 % CI | Constraint |
|---|---|---|---|
| Top | 96.3 % | [84.7, 107.9] % | Free |
| Bottom | 7.1 % | [5.7, 8.5] % | Free |
| IC50 | 3.25 µM | [2.56, 4.12] µM | Free |
| pIC50 | 5.49 | ± 0.05 (SE) | Derived |
| Hill slope (nH) | 1.51 | [1.17, 1.85] | Free |
R² 0.994RMSE 2.0 %df 16Fit engine 1.2.0 · 20 / 20 points
Review decision
IC50
3.25 µM
95 % CI 2.56–4.12 µM · pIC50 5.49
Rejected: Signal drift across the plate, replicates disagree
History
append-only- v1 auto-fit createdsystem · 2026-11-04 17:05:40 UTCFit engine 1.2.0 · 20 / 20 points · incompleteTop
- Rejectedmlaurent · 2026-11-04 18:10:00 UTC“Signal drift across the plate, replicates disagree” · v1 · auto-fit