AFN-000514-01 · EGFR WT
Approved · lockedCRV-2026-00442-024 · v1 · auto-fitPlates PL-2026-0442, PL-2026-0443 (n = 2 replicates)4PL · Levenberg–Marquardt · unweighted, log₁₀[C]
Curve 6 / 10 in batch
Response vs concentration
- Rep. 1 · PL-0442
- Rep. 2 · PL-0443
- Masked
- IC50 95 % CI
Concentrations: 10-point, 1:3, 0.508 nM – 10.0 µMPoints used 20 / 20
Fit parameters — v1 · auto-fit
y = Bottom + (Top − Bottom) / (1 + 10^((log IC50 − x) · nH))| Parameter | Estimate | 95 % CI | Constraint |
|---|---|---|---|
| Top | 100.9 % | [96.0, 105.9] % | Free |
| Bottom | 6.4 % | [5.5, 7.4] % | Free |
| IC50 | 1.57 µM | [1.38, 1.78] µM | Free |
| pIC50 | 5.81 | ± 0.03 (SE) | Derived |
| Hill slope (nH) | 1.16 | [1.04, 1.28] | Free |
R² 0.998RMSE 1.2 %df 16Fit engine 1.2.0 · 20 / 20 points
Review decision
IC50
1.57 µM
95 % CI 1.38–1.78 µM · pIC50 5.81
Electronically signed
mlaurent (mlaurent)
2026-11-04 17:40:00 UTC
Meaning: Approved — result valid for SAR use
record sha256 afbf…6c05
This result is immutable. A correction creates a new version with a mandatory reason and its own signature.
History
append-only- v1 auto-fit createdsystem · 2026-11-04 17:05:40 UTCFit engine 1.2.0 · 20 / 20 points · incompleteTop
- Re-authenticatedmlaurent · 2026-11-04 17:40:00 UTCPassword verified for signing
- Signed — Approvedmlaurent · 2026-11-04 17:40:00 UTCMeaning: Approved — result valid for SAR use · v1 · auto-fit · record hash sha256 afbf…6c05