AFN-001663-01 · EGFR WT
RejectedCRV-2026-00450-029 · v1 · auto-fitPlates PL-2026-0450, PL-2026-0451 (n = 2 replicates)4PL · Levenberg–Marquardt · unweighted, log₁₀[C]
Curve 9 / 10 in batch
Response vs concentration
- Rep. 1 · PL-0450
- Rep. 2 · PL-0451
- Masked
- IC50 95 % CI
Concentrations: 10-point, 1:3, 0.508 nM – 10.0 µMPoints used 20 / 20
Fit parameters — v1 · auto-fit
y = Bottom + (Top − Bottom) / (1 + 10^((log IC50 − x) · nH))| Parameter | Estimate | 95 % CI | Constraint |
|---|---|---|---|
| Top | 133.3 % | [−2.0, 268.7] % | Free |
| Bottom | 3.2 % | [1.5, 5.0] % | Free |
| IC50 | 10.0 µM | — | Free |
| pIC50 | 4.93 | ± 0.42 (SE) | Derived |
| Hill slope (nH) | 0.96 | [0.53, 1.39] | Free |
R² 0.986RMSE 2.2 %df 16Fit engine 1.2.0 · 20 / 20 points
Review decision
IC50
> 10.0 µM
pIC50 4.93
Rejected: Compound precipitated at the top two concentrations
History
append-only- v1 auto-fit createdsystem · 2026-11-04 17:05:40 UTCFit engine 1.2.0 · 20 / 20 points · aboveRange
- Rejectedmlaurent · 2026-11-04 18:10:00 UTC“Compound precipitated at the top two concentrations” · v1 · auto-fit