AFN-001733-01 · EGFR WT
RejectedCRV-2026-00452-006 · v1 · auto-fitPlates PL-2026-0452, PL-2026-0453 (n = 2 replicates)4PL · Levenberg–Marquardt · unweighted, log₁₀[C]
Curve 8 / 10 in batch
Response vs concentration
- Rep. 1 · PL-0452
- Rep. 2 · PL-0453
- Masked
- IC50 95 % CI
Concentrations: 10-point, 1:3, 0.508 nM – 10.0 µMPoints used 20 / 20
Fit parameters — v1 · auto-fit
y = Bottom + (Top − Bottom) / (1 + 10^((log IC50 − x) · nH))| Parameter | Estimate | 95 % CI | Constraint |
|---|---|---|---|
| Top | 99.8 % | [93.1, 106.5] % | Free |
| Bottom | 5.7 % | [4.7, 6.7] % | Free |
| IC50 | 1.66 µM | [1.36, 2.03] µM | Free |
| pIC50 | 5.78 | ± 0.04 (SE) | Derived |
| Hill slope (nH) | 0.89 | [0.80, 0.99] | Free |
R² 0.998RMSE 1.1 %df 16Fit engine 1.2.0 · 20 / 20 points
Review decision
IC50
1.66 µM
95 % CI 1.36–2.03 µM · pIC50 5.78
Rejected: Curve does not plateau — retest over a wider range
History
append-only- v1 auto-fit createdsystem · 2026-11-04 17:05:40 UTCFit engine 1.2.0 · 20 / 20 points · incompleteTop
- Rejectedmlaurent · 2026-11-04 18:10:00 UTC“Curve does not plateau — retest over a wider range” · v1 · auto-fit