AFN-001781-01 · EGFR WT
RejectedCRV-2026-00452-013 · v1 · auto-fitPlates PL-2026-0452, PL-2026-0453 (n = 2 replicates)4PL · Levenberg–Marquardt · unweighted, log₁₀[C]
Curve 5 / 10 in batch
Response vs concentration
- Rep. 1 · PL-0452
- Rep. 2 · PL-0453
- Masked
- IC50 95 % CI
Concentrations: 10-point, 1:3, 0.508 nM – 10.0 µMPoints used 20 / 20
Fit parameters — v1 · auto-fit
y = Bottom + (Top − Bottom) / (1 + 10^((log IC50 − x) · nH))| Parameter | Estimate | 95 % CI | Constraint |
|---|---|---|---|
| Top | 99.7 % | [88.7, 110.7] % | Free |
| Bottom | 3.7 % | [1.5, 5.9] % | Free |
| IC50 | 1.13 µM | [0.797, 1.60] µM | Free |
| pIC50 | 5.95 | ± 0.07 (SE) | Derived |
| Hill slope (nH) | 0.85 | [0.68, 1.02] | Free |
R² 0.994RMSE 2.2 %df 16Fit engine 1.2.0 · 20 / 20 points
Review decision
IC50
1.13 µM
95 % CI 0.797–1.60 µM · pIC50 5.95
Rejected: Curve does not plateau — retest over a wider range
History
append-only- v1 auto-fit createdsystem · 2026-11-04 17:05:40 UTCFit engine 1.2.0 · 20 / 20 points · incompleteTop
- Rejectedmlaurent · 2026-11-04 18:10:00 UTC“Curve does not plateau — retest over a wider range” · v1 · auto-fit