AFN-002147-01 · EGFR WT
RejectedCRV-2026-00454-027 · v1 · auto-fitPlates PL-2026-0454, PL-2026-0455 (n = 2 replicates)4PL · Levenberg–Marquardt · unweighted, log₁₀[C]
Curve 1 / 10 in batch
Response vs concentration
- Rep. 1 · PL-0454
- Rep. 2 · PL-0455
- Masked
- IC50 95 % CI
Concentrations: 10-point, 1:3, 0.508 nM – 10.0 µMPoints used 20 / 20
Fit parameters — v1 · auto-fit
y = Bottom + (Top − Bottom) / (1 + 10^((log IC50 − x) · nH))| Parameter | Estimate | 95 % CI | Constraint |
|---|---|---|---|
| Top | 102.2 % | [91.1, 113.3] % | Free |
| Bottom | 5.9 % | [4.1, 7.6] % | Free |
| IC50 | 1.96 µM | [1.48, 2.58] µM | Free |
| pIC50 | 5.71 | ± 0.06 (SE) | Derived |
| Hill slope (nH) | 1.14 | [0.91, 1.38] | Free |
R² 0.994RMSE 2.2 %df 16Fit engine 1.2.0 · 20 / 20 points
Review decision
IC50
1.96 µM
95 % CI 1.48–2.58 µM · pIC50 5.71
Rejected: Signal drift across the plate, replicates disagree
History
append-only- v1 auto-fit createdsystem · 2026-11-04 17:05:40 UTCFit engine 1.2.0 · 20 / 20 points · incompleteTop
- Rejectedmlaurent · 2026-11-04 18:10:00 UTC“Signal drift across the plate, replicates disagree” · v1 · auto-fit