AFN-002526-01 · EGFR WT
RejectedCRV-2026-00458-021 · v1 · auto-fitPlates PL-2026-0458, PL-2026-0459 (n = 2 replicates)4PL · Levenberg–Marquardt · unweighted, log₁₀[C]
Curve 9 / 10 in batch
Response vs concentration
- Rep. 1 · PL-0458
- Rep. 2 · PL-0459
- Masked
- IC50 95 % CI
Concentrations: 10-point, 1:3, 0.508 nM – 10.0 µMPoints used 20 / 20
Fit parameters — v1 · auto-fit
y = Bottom + (Top − Bottom) / (1 + 10^((log IC50 − x) · nH))| Parameter | Estimate | 95 % CI | Constraint |
|---|---|---|---|
| Top | 102.6 % | [93.8, 111.3] % | Free |
| Bottom | 5.9 % | [4.5, 7.3] % | Free |
| IC50 | 1.99 µM | [1.62, 2.46] µM | Free |
| pIC50 | 5.70 | ± 0.04 (SE) | Derived |
| Hill slope (nH) | 1.22 | [1.01, 1.42] | Free |
R² 0.996RMSE 1.9 %df 16Fit engine 1.2.0 · 20 / 20 points
Review decision
IC50
1.99 µM
95 % CI 1.62–2.46 µM · pIC50 5.70
Rejected: Signal drift across the plate, replicates disagree
History
append-only- v1 auto-fit createdsystem · 2026-11-04 17:05:40 UTCFit engine 1.2.0 · 20 / 20 points · incompleteTop
- Rejectedmlaurent · 2026-11-04 18:10:00 UTC“Signal drift across the plate, replicates disagree” · v1 · auto-fit