AFN-002733-01 · EGFR WT
RejectedCRV-2026-00460-011 · v1 · auto-fitPlates PL-2026-0460, PL-2026-0461 (n = 2 replicates)4PL · Levenberg–Marquardt · unweighted, log₁₀[C]
Curve 1 / 10 in batch
Response vs concentration
- Rep. 1 · PL-0460
- Rep. 2 · PL-0461
- Masked
- IC50 95 % CI
Concentrations: 10-point, 1:3, 0.508 nM – 10.0 µMPoints used 20 / 20
Fit parameters — v1 · auto-fit
y = Bottom + (Top − Bottom) / (1 + 10^((log IC50 − x) · nH))| Parameter | Estimate | 95 % CI | Constraint |
|---|---|---|---|
| Top | 101.7 % | [96.1, 107.3] % | Free |
| Bottom | 5.7 % | [4.4, 7.0] % | Free |
| IC50 | 1.02 µM | [0.865, 1.21] µM | Free |
| pIC50 | 5.99 | ± 0.03 (SE) | Derived |
| Hill slope (nH) | 1.00 | [0.87, 1.12] | Free |
R² 0.998RMSE 1.5 %df 16Fit engine 1.2.0 · 20 / 20 points
Review decision
IC50
1.02 µM
95 % CI 0.865–1.21 µM · pIC50 5.99
Rejected: Compound precipitated at the top two concentrations
History
append-only- v1 auto-fit createdsystem · 2026-11-04 17:05:40 UTCFit engine 1.2.0 · 20 / 20 points · no flags
- Rejectedmlaurent · 2026-11-04 18:10:00 UTC“Compound precipitated at the top two concentrations” · v1 · auto-fit