AFN-003284-01 · EGFR WT
RejectedCRV-2026-00464-017 · v1 · auto-fitPlates PL-2026-0464, PL-2026-0465 (n = 2 replicates)4PL · Levenberg–Marquardt · unweighted, log₁₀[C]
Curve 1 / 10 in batch
Response vs concentration
- Rep. 1 · PL-0464
- Rep. 2 · PL-0465
- Masked
- IC50 95 % CI
Concentrations: 10-point, 1:3, 0.508 nM – 10.0 µMPoints used 20 / 20
Fit parameters — v1 · auto-fit
y = Bottom + (Top − Bottom) / (1 + 10^((log IC50 − x) · nH))| Parameter | Estimate | 95 % CI | Constraint |
|---|---|---|---|
| Top | 99.6 % | [72.7, 126.5] % | Free |
| Bottom | 5.4 % | [3.4, 7.4] % | Free |
| IC50 | 3.35 µM | [1.54, 7.27] µM | Free |
| pIC50 | 5.48 | ± 0.16 (SE) | Derived |
| Hill slope (nH) | 0.84 | [0.60, 1.08] | Free |
R² 0.991RMSE 2.2 %df 16Fit engine 1.2.0 · 20 / 20 points
Review decision
IC50
3.35 µM
95 % CI 1.54–7.27 µM · pIC50 5.48
Rejected: Signal drift across the plate, replicates disagree
History
append-only- v1 auto-fit createdsystem · 2026-11-04 17:05:40 UTCFit engine 1.2.0 · 20 / 20 points · incompleteTop
- Rejectedmlaurent · 2026-11-04 18:10:00 UTC“Signal drift across the plate, replicates disagree” · v1 · auto-fit