AFN-003522-01 · EGFR WT
RejectedCRV-2026-00466-031 · v1 · auto-fitPlates PL-2026-0466, PL-2026-0467 (n = 2 replicates)4PL · Levenberg–Marquardt · unweighted, log₁₀[C]
Curve 7 / 10 in batch
Response vs concentration
- Rep. 1 · PL-0466
- Rep. 2 · PL-0467
- Masked
- IC50 95 % CI
Concentrations: 10-point, 1:3, 0.508 nM – 10.0 µMPoints used 20 / 20
Fit parameters — v1 · auto-fit
y = Bottom + (Top − Bottom) / (1 + 10^((log IC50 − x) · nH))| Parameter | Estimate | 95 % CI | Constraint |
|---|---|---|---|
| Top | 99.3 % | [98.4, 100.1] % | Free |
| Bottom | 1.5 % | [−0.7, 3.8] % | Free |
| IC50 | 9.70 nM | [9.01, 10.4] nM | Free |
| pIC50 | 8.01 | ± 0.02 (SE) | Derived |
| Hill slope (nH) | 1.03 | [0.97, 1.10] | Free |
R² 0.999RMSE 0.9 %df 16Fit engine 1.2.0 · 20 / 20 points
Review decision
IC50
9.70 nM
95 % CI 9.01–10.4 nM · pIC50 8.01
Rejected: Curve does not plateau — retest over a wider range
History
append-only- v1 auto-fit createdsystem · 2026-11-04 17:05:40 UTCFit engine 1.2.0 · 20 / 20 points · no flags
- Rejectedmlaurent · 2026-11-04 18:10:00 UTC“Curve does not plateau — retest over a wider range” · v1 · auto-fit