AFN-003744-01 · EGFR WT
RejectedCRV-2026-00468-030 · v1 · auto-fitPlates PL-2026-0468, PL-2026-0469 (n = 2 replicates)4PL · Levenberg–Marquardt · unweighted, log₁₀[C]
Curve 8 / 10 in batch
Response vs concentration
- Rep. 1 · PL-0468
- Rep. 2 · PL-0469
- Masked
- IC50 95 % CI
Concentrations: 10-point, 1:3, 0.508 nM – 10.0 µMPoints used 20 / 20
Fit parameters — v1 · auto-fit
y = Bottom + (Top − Bottom) / (1 + 10^((log IC50 − x) · nH))| Parameter | Estimate | 95 % CI | Constraint |
|---|---|---|---|
| Top | 100.4 % | [99.0, 101.8] % | Free |
| Bottom | 1.5 % | [−2.8, 5.8] % | Free |
| IC50 | 6.69 nM | [5.90, 7.58] nM | Free |
| pIC50 | 8.17 | ± 0.03 (SE) | Derived |
| Hill slope (nH) | 1.16 | [1.02, 1.30] | Free |
R² 0.998RMSE 1.7 %df 16Fit engine 1.2.0 · 20 / 20 points
Review decision
IC50
6.69 nM
95 % CI 5.90–7.58 nM · pIC50 8.17
Rejected: Signal drift across the plate, replicates disagree
History
append-only- v1 auto-fit createdsystem · 2026-11-04 17:05:40 UTCFit engine 1.2.0 · 20 / 20 points · no flags
- Rejectedmlaurent · 2026-11-04 18:10:00 UTC“Signal drift across the plate, replicates disagree” · v1 · auto-fit