AFN-004255-01 · EGFR WT
RejectedCRV-2026-00474-005 · v1 · auto-fitPlates PL-2026-0474, PL-2026-0475 (n = 2 replicates)4PL · Levenberg–Marquardt · unweighted, log₁₀[C]
Curve 9 / 10 in batch
Response vs concentration
- Rep. 1 · PL-0474
- Rep. 2 · PL-0475
- Masked
- IC50 95 % CI
Concentrations: 10-point, 1:3, 0.508 nM – 10.0 µMPoints used 20 / 20
Fit parameters — v1 · auto-fit
y = Bottom + (Top − Bottom) / (1 + 10^((log IC50 − x) · nH))| Parameter | Estimate | 95 % CI | Constraint |
|---|---|---|---|
| Top | 100.9 % | [100.1, 101.7] % | Free |
| Bottom | 10.8 % | [8.9, 12.8] % | Free |
| IC50 | 9.11 nM | [8.51, 9.75] nM | Free |
| pIC50 | 8.04 | ± 0.01 (SE) | Derived |
| Hill slope (nH) | 1.13 | [1.06, 1.21] | Free |
R² 0.999RMSE 0.9 %df 16Fit engine 1.2.0 · 20 / 20 points
Review decision
IC50
9.11 nM
95 % CI 8.51–9.75 nM · pIC50 8.04
Rejected: Curve does not plateau — retest over a wider range
History
append-only- v1 auto-fit createdsystem · 2026-11-04 17:05:40 UTCFit engine 1.2.0 · 20 / 20 points · no flags
- Rejectedmlaurent · 2026-11-04 18:10:00 UTC“Curve does not plateau — retest over a wider range” · v1 · auto-fit