AFN-000213-01 · H1975 cells

Approved · locked

CRV-2026-00478-001 · v1 · auto-fitPlates PL-2026-0478 (n = 1 replicates)4PL · Levenberg–Marquardt · unweighted, log₁₀[C]

Curve 7 / 10 in batch

Response vs concentration

Concentrations: 8-point, 1:3, 4.57 nM – 10.0 µMPoints used 8 / 8

y = Bottom + (Top − Bottom) / (1 + 10^((log IC50 − x) · nH))

Parameter	Estimate	95 % CI	Constraint
Top	165.7 %	[−20.7, 352.1] %	Free
Bottom	−5.0 %	[−10.6, 0.6] %	Free
IC50	10.0 µM	—	Free
pIC50	4.94	± 0.49 (SE)	Derived
Hill slope (nH)	0.65	[0.28, 1.02]	Free

R² 0.997RMSE 1.4 %df 4Fit engine 1.2.0 · 8 / 8 points

IC50

> 10.0 µM

pIC50 4.94

Electronically signed

mlaurent (mlaurent)

2026-11-04 17:40:00 UTC

Meaning: Approved — result valid for SAR use

record sha256 203e…2162

This result is immutable. A correction creates a new version with a mandatory reason and its own signature.

append-only

v1 auto-fit createdsystem · 2026-11-04 17:05:40 UTCFit engine 1.2.0 · 8 / 8 points · aboveRange
Re-authenticatedmlaurent · 2026-11-04 17:40:00 UTCPassword verified for signing
Signed — Approvedmlaurent · 2026-11-04 17:40:00 UTCMeaning: Approved — result valid for SAR use · v1 · auto-fit · record hash sha256 203e…2162