AFN-001379-01 · H1975 cells
Approved · lockedCRV-2026-00495-004 · v1 · auto-fitPlates PL-2026-0495 (n = 1 replicates)4PL · Levenberg–Marquardt · unweighted, log₁₀[C]
Curve 6 / 10 in batch
Response vs concentration
- Rep. 1 · PL-0495
- Masked
- IC50 95 % CI
Concentrations: 8-point, 1:3, 4.57 nM – 10.0 µMPoints used 8 / 8
Fit parameters — v1 · auto-fit
y = Bottom + (Top − Bottom) / (1 + 10^((log IC50 − x) · nH))| Parameter | Estimate | 95 % CI | Constraint |
|---|---|---|---|
| Top | 101.3 % | [99.2, 103.5] % | Free |
| Bottom | 8.7 % | [6.7, 10.8] % | Free |
| IC50 | 246 nM | [225, 268] nM | Free |
| pIC50 | 6.61 | ± 0.01 (SE) | Derived |
| Hill slope (nH) | 1.31 | [1.17, 1.45] | Free |
R² 1.000RMSE 0.6 %df 4Fit engine 1.2.0 · 8 / 8 points
Review decision
IC50
246 nM
95 % CI 225–268 nM · pIC50 6.61
Electronically signed
mlaurent (mlaurent)
2026-11-04 17:40:00 UTC
Meaning: Approved — result valid for SAR use
record sha256 953b…c43a
This result is immutable. A correction creates a new version with a mandatory reason and its own signature.
History
append-only- v1 auto-fit createdsystem · 2026-11-04 17:05:40 UTCFit engine 1.2.0 · 8 / 8 points · no flags
- Re-authenticatedmlaurent · 2026-11-04 17:40:00 UTCPassword verified for signing
- Signed — Approvedmlaurent · 2026-11-04 17:40:00 UTCMeaning: Approved — result valid for SAR use · v1 · auto-fit · record hash sha256 953b…c43a