AFN-001664-01 · H1975 cells
Approved · lockedCRV-2026-00499-006 · v1 · auto-fitPlates PL-2026-0499 (n = 1 replicates)4PL · Levenberg–Marquardt · unweighted, log₁₀[C]
Curve 10 / 10 in batch
Response vs concentration
- Rep. 1 · PL-0499
- Masked
- IC50 95 % CI
Concentrations: 8-point, 1:3, 4.57 nM – 10.0 µMPoints used 8 / 8
Fit parameters — v1 · auto-fit
y = Bottom + (Top − Bottom) / (1 + 10^((log IC50 − x) · nH))| Parameter | Estimate | 95 % CI | Constraint |
|---|---|---|---|
| Top | 99.9 % | [94.2, 105.6] % | Free |
| Bottom | 7.5 % | [2.1, 12.9] % | Free |
| IC50 | 236 nM | [186, 299] nM | Free |
| pIC50 | 6.63 | ± 0.04 (SE) | Derived |
| Hill slope (nH) | 0.95 | [0.73, 1.18] | Free |
R² 0.999RMSE 1.2 %df 4Fit engine 1.2.0 · 8 / 8 points
Review decision
IC50
236 nM
95 % CI 186–299 nM · pIC50 6.63
Electronically signed
mlaurent (mlaurent)
2026-11-04 17:40:00 UTC
Meaning: Approved — result valid for SAR use
record sha256 1a6d…d513
This result is immutable. A correction creates a new version with a mandatory reason and its own signature.
History
append-only- v1 auto-fit createdsystem · 2026-11-04 17:05:40 UTCFit engine 1.2.0 · 8 / 8 points · no flags
- Re-authenticatedmlaurent · 2026-11-04 17:40:00 UTCPassword verified for signing
- Signed — Approvedmlaurent · 2026-11-04 17:40:00 UTCMeaning: Approved — result valid for SAR use · v1 · auto-fit · record hash sha256 1a6d…d513