AFN-002212-01 · H1975 cells
Approved · lockedCRV-2026-00508-003 · v1 · auto-fitPlates PL-2026-0508 (n = 1 replicates)4PL · Levenberg–Marquardt · unweighted, log₁₀[C]
Curve 9 / 10 in batch
Response vs concentration
- Rep. 1 · PL-0508
- Masked
- IC50 95 % CI
Concentrations: 8-point, 1:3, 4.57 nM – 10.0 µMPoints used 8 / 8
Fit parameters — v1 · auto-fit
y = Bottom + (Top − Bottom) / (1 + 10^((log IC50 − x) · nH))| Parameter | Estimate | 95 % CI | Constraint |
|---|---|---|---|
| Top | 100.0 % | [97.4, 102.6] % | Free |
| Bottom | 5.3 % | [2.4, 8.2] % | Free |
| IC50 | 179 nM | [160, 201] nM | Free |
| pIC50 | 6.75 | ± 0.02 (SE) | Derived |
| Hill slope (nH) | 1.15 | [1.00, 1.29] | Free |
R² 1.000RMSE 0.7 %df 4Fit engine 1.2.0 · 8 / 8 points
Review decision
IC50
179 nM
95 % CI 160–201 nM · pIC50 6.75
Electronically signed
mlaurent (mlaurent)
2026-11-04 17:40:00 UTC
Meaning: Approved — result valid for SAR use
record sha256 ff85…dd57
This result is immutable. A correction creates a new version with a mandatory reason and its own signature.
History
append-only- v1 auto-fit createdsystem · 2026-11-04 17:05:40 UTCFit engine 1.2.0 · 8 / 8 points · no flags
- Re-authenticatedmlaurent · 2026-11-04 17:40:00 UTCPassword verified for signing
- Signed — Approvedmlaurent · 2026-11-04 17:40:00 UTCMeaning: Approved — result valid for SAR use · v1 · auto-fit · record hash sha256 ff85…dd57