AFN-002410-01 · H1975 cells
RejectedCRV-2026-00512-006 · v1 · auto-fitPlates PL-2026-0512 (n = 1 replicates)4PL · Levenberg–Marquardt · unweighted, log₁₀[C]
Curve 4 / 10 in batch
Response vs concentration
- Rep. 1 · PL-0512
- Masked
- IC50 95 % CI
Concentrations: 8-point, 1:3, 4.57 nM – 10.0 µMPoints used 8 / 8
Fit parameters — v1 · auto-fit
y = Bottom + (Top − Bottom) / (1 + 10^((log IC50 − x) · nH))| Parameter | Estimate | 95 % CI | Constraint |
|---|---|---|---|
| Top | 101.0 % | [99.3, 102.6] % | Free |
| Bottom | 9.1 % | [5.5, 12.8] % | Free |
| IC50 | 72.1 nM | [64.1, 81.2] nM | Free |
| pIC50 | 7.14 | ± 0.02 (SE) | Derived |
| Hill slope (nH) | 0.91 | [0.82, 1.00] | Free |
R² 1.000RMSE 0.5 %df 4Fit engine 1.2.0 · 8 / 8 points
Review decision
IC50
72.1 nM
95 % CI 64.1–81.2 nM · pIC50 7.14
Rejected: Signal drift across the plate, replicates disagree
History
append-only- v1 auto-fit createdsystem · 2026-11-04 17:05:40 UTCFit engine 1.2.0 · 8 / 8 points · no flags
- Rejectedmlaurent · 2026-11-04 18:10:00 UTC“Signal drift across the plate, replicates disagree” · v1 · auto-fit