AFN-003018-01 · H1975 cells

Approved · locked

CRV-2026-00522-003 · v1 · auto-fitPlates PL-2026-0522 (n = 1 replicates)4PL · Levenberg–Marquardt · unweighted, log₁₀[C]

Curve 1 / 10 in batch

Response vs concentration

Concentrations: 8-point, 1:3, 4.57 nM – 10.0 µMPoints used 8 / 8

y = Bottom + (Top − Bottom) / (1 + 10^((log IC50 − x) · nH))

Parameter	Estimate	95 % CI	Constraint
Top	102.5 %	[97.7, 107.2] %	Free
Bottom	−0.9 %	[−13.2, 11.3] %	Free
IC50	59.4 nM	[41.9, 84.3] nM	Free
pIC50	7.23	± 0.05 (SE)	Derived
Hill slope (nH)	0.86	[0.63, 1.10]	Free

R² 0.999RMSE 1.3 %df 4Fit engine 1.2.0 · 8 / 8 points

IC50

59.4 nM

95 % CI 41.9–84.3 nM · pIC50 7.23

Electronically signed

mlaurent (mlaurent)

2026-11-04 17:40:00 UTC

Meaning: Approved — result valid for SAR use

record sha256 3b4c…65b7

This result is immutable. A correction creates a new version with a mandatory reason and its own signature.

append-only

v1 auto-fit createdsystem · 2026-11-04 17:05:40 UTCFit engine 1.2.0 · 8 / 8 points · no flags
Re-authenticatedmlaurent · 2026-11-04 17:40:00 UTCPassword verified for signing
Signed — Approvedmlaurent · 2026-11-04 17:40:00 UTCMeaning: Approved — result valid for SAR use · v1 · auto-fit · record hash sha256 3b4c…65b7