■ Demo— not a validated GxP system — synthetic data

Affinis

PL-2026-0464 · EGFR WT — TR-FRET kinase assay

QC passed

384-well · ASY-EGFR-WT-02 v1Read 2026-11-04 16:42:19 UTCInstrument EnVision-02 (mock import)Raw file sha256 dcf4…8e15Imported by mlaurent

6,04549,249RFU · raw
HC
Cmpd A · 10-pt, 1:3 from 10 µM
Cmpd B · 10-pt, 1:3 from 10 µM
LC
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Masked — excluded from QC and fits, retained

Edge effect (HC, outer rows vs interior): +3.7 %

384 wells · 0 masked

Plate QC

rules QC-STD v3
Z′-factor0.86accept ≥ 0.50
Signal window (SW)
21.1≥ 2.0
Signal / background
6.86≥ 3.0
CV high control
3.5 %≤ 10 %
CV low control
3.5 %≤ 10 %
Masked wells
0.0 %≤ 5 %

Plates failing any rule are rejected automatically. Rejection can be overridden only by a signed exception.

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Timestamp (UTC)ActorEventDetail
2026-11-04 16:42:19 UTCsystemQC evaluated — passedQC-STD v3 · all rules met
2026-11-04 16:42:19 UTCsystemNormalization v1 computedμHC, μLC from cols 1–2 / 23–24 (n = 32 each)
2026-11-04 16:42:19 UTCsystemRaw data importedEnVision-02 export PL-2026-0464.csv · 384 wells · sha256 dcf4…8e15 · stored write-once