AFN-000454-01 · EGFR L858R/T790M
Approved · lockedCRV-2026-00403-012 · v1 · auto-fitPlates PL-2026-0403, PL-2026-0404 (n = 2 replicates)4PL · Levenberg–Marquardt · unweighted, log₁₀[C]
Curve 4 / 10 in batch
Response vs concentration
- Rep. 1 · PL-0403
- Rep. 2 · PL-0404
- Masked
- IC50 95 % CI
Concentrations: 10-point, 1:3, 0.508 nM – 10.0 µMPoints used 20 / 20
Fit parameters — v1 · auto-fit
y = Bottom + (Top − Bottom) / (1 + 10^((log IC50 − x) · nH))| Parameter | Estimate | 95 % CI | Constraint |
|---|---|---|---|
| Top | 101.0 % | [100.1, 101.9] % | Free |
| Bottom | 4.9 % | [2.4, 7.5] % | Free |
| IC50 | 7.55 nM | [6.98, 8.17] nM | Free |
| pIC50 | 8.12 | ± 0.02 (SE) | Derived |
| Hill slope (nH) | 1.09 | [1.01, 1.17] | Free |
R² 0.999RMSE 1.0 %df 16Fit engine 1.2.0 · 20 / 20 points
Review decision
IC50
7.55 nM
95 % CI 6.98–8.17 nM · pIC50 8.12
Electronically signed
mlaurent (mlaurent)
2026-11-04 17:40:00 UTC
Meaning: Approved — result valid for SAR use
record sha256 f8b0…22e4
This result is immutable. A correction creates a new version with a mandatory reason and its own signature.
History
append-only- v1 auto-fit createdsystem · 2026-11-04 17:05:40 UTCFit engine 1.2.0 · 20 / 20 points · no flags
- Re-authenticatedmlaurent · 2026-11-04 17:40:00 UTCPassword verified for signing
- Signed — Approvedmlaurent · 2026-11-04 17:40:00 UTCMeaning: Approved — result valid for SAR use · v1 · auto-fit · record hash sha256 f8b0…22e4