AFN-000488-01 · EGFR L858R/T790M
RejectedCRV-2026-00403-018 · v1 · auto-fitPlates PL-2026-0403, PL-2026-0404 (n = 2 replicates)4PL · Levenberg–Marquardt · unweighted, log₁₀[C]
Curve 10 / 10 in batch
Response vs concentration
- Rep. 1 · PL-0403
- Rep. 2 · PL-0404
- Masked
- IC50 95 % CI
Concentrations: 10-point, 1:3, 0.508 nM – 10.0 µMPoints used 20 / 20
Fit parameters — v1 · auto-fit
y = Bottom + (Top − Bottom) / (1 + 10^((log IC50 − x) · nH))| Parameter | Estimate | 95 % CI | Constraint |
|---|---|---|---|
| Top | 100.4 % | [98.2, 102.6] % | Free |
| Bottom | 7.5 % | [5.3, 9.7] % | Free |
| IC50 | 71.3 nM | [63.5, 80.1] nM | Free |
| pIC50 | 7.15 | ± 0.02 (SE) | Derived |
| Hill slope (nH) | 0.89 | [0.80, 0.98] | Free |
R² 0.998RMSE 1.5 %df 16Fit engine 1.2.0 · 20 / 20 points
Review decision
IC50
71.3 nM
95 % CI 63.5–80.1 nM · pIC50 7.15
Rejected: Compound precipitated at the top two concentrations
History
append-only- v1 auto-fit createdsystem · 2026-11-04 17:05:40 UTCFit engine 1.2.0 · 20 / 20 points · no flags
- Rejectedmlaurent · 2026-11-04 18:10:00 UTC“Compound precipitated at the top two concentrations” · v1 · auto-fit