AFN-002283-01 · EGFR L858R/T790M
RejectedCRV-2026-00417-018 · v1 · auto-fitPlates PL-2026-0417, PL-2026-0419 (n = 2 replicates)4PL · Levenberg–Marquardt · unweighted, log₁₀[C]
Curve 4 / 10 in batch
Response vs concentration
- Rep. 1 · PL-0417
- Rep. 2 · PL-0419
- Masked
- IC50 95 % CI
Concentrations: 10-point, 1:3, 0.508 nM – 10.0 µMPoints used 20 / 20
Fit parameters — v1 · auto-fit
y = Bottom + (Top − Bottom) / (1 + 10^((log IC50 − x) · nH))| Parameter | Estimate | 95 % CI | Constraint |
|---|---|---|---|
| Top | 100.4 % | [97.9, 102.9] % | Free |
| Bottom | 6.6 % | [4.7, 8.6] % | Free |
| IC50 | 128 nM | [115, 143] nM | Free |
| pIC50 | 6.89 | ± 0.02 (SE) | Derived |
| Hill slope (nH) | 1.09 | [0.97, 1.21] | Free |
R² 0.998RMSE 1.8 %df 16Fit engine 1.2.0 · 20 / 20 points
Review decision
IC50
128 nM
95 % CI 115–143 nM · pIC50 6.89
Rejected: Compound precipitated at the top two concentrations
History
append-only- v1 auto-fit createdsystem · 2026-11-04 17:05:40 UTCFit engine 1.2.0 · 20 / 20 points · no flags
- Rejectedmlaurent · 2026-11-04 18:10:00 UTC“Compound precipitated at the top two concentrations” · v1 · auto-fit