AFN-002312-01 · EGFR L858R/T790M
RejectedCRV-2026-00417-022 · v1 · auto-fitPlates PL-2026-0417, PL-2026-0419 (n = 2 replicates)4PL · Levenberg–Marquardt · unweighted, log₁₀[C]
Curve 8 / 10 in batch
Response vs concentration
- Rep. 1 · PL-0417
- Rep. 2 · PL-0419
- Masked
- IC50 95 % CI
Concentrations: 10-point, 1:3, 0.508 nM – 10.0 µMPoints used 20 / 20
Fit parameters — v1 · auto-fit
y = Bottom + (Top − Bottom) / (1 + 10^((log IC50 − x) · nH))| Parameter | Estimate | 95 % CI | Constraint |
|---|---|---|---|
| Top | 99.9 % | [93.1, 106.6] % | Free |
| Bottom | 3.5 % | [−5.3, 12.4] % | Free |
| IC50 | 38.0 nM | [25.7, 56.1] nM | Free |
| pIC50 | 7.42 | ± 0.08 (SE) | Derived |
| Hill slope (nH) | 1.01 | [0.64, 1.38] | Free |
R² 0.977RMSE 5.9 %df 16Fit engine 1.2.0 · 20 / 20 points
Review decision
IC50
38.0 nM
95 % CI 25.7–56.1 nM · pIC50 7.42
Rejected: Compound precipitated at the top two concentrations
History
append-only- v1 auto-fit createdsystem · 2026-11-04 17:05:40 UTCFit engine 1.2.0 · 20 / 20 points · no flags
- Rejectedmlaurent · 2026-11-04 18:10:00 UTC“Compound precipitated at the top two concentrations” · v1 · auto-fit