AFN-002258-01 · EGFR WT
RejectedCRV-2026-00456-015 · v1 · auto-fitPlates PL-2026-0456, PL-2026-0457 (n = 2 replicates)4PL · Levenberg–Marquardt · unweighted, log₁₀[C]
Curve 1 / 10 in batch
Response vs concentration
- Rep. 1 · PL-0456
- Rep. 2 · PL-0457
- Masked
- IC50 95 % CI
Concentrations: 10-point, 1:3, 0.508 nM – 10.0 µMPoints used 20 / 20
Fit parameters — v1 · auto-fit
y = Bottom + (Top − Bottom) / (1 + 10^((log IC50 − x) · nH))| Parameter | Estimate | 95 % CI | Constraint |
|---|---|---|---|
| Top | 102.5 % | [96.9, 108.1] % | Free |
| Bottom | 4.4 % | [3.0, 5.9] % | Free |
| IC50 | 814 nM | [683, 970] nM | Free |
| pIC50 | 6.09 | ± 0.04 (SE) | Derived |
| Hill slope (nH) | 0.92 | [0.81, 1.03] | Free |
R² 0.998RMSE 1.6 %df 16Fit engine 1.2.0 · 20 / 20 points
Review decision
IC50
814 nM
95 % CI 683–970 nM · pIC50 6.09
Rejected: Compound precipitated at the top two concentrations
History
append-only- v1 auto-fit createdsystem · 2026-11-04 17:05:40 UTCFit engine 1.2.0 · 20 / 20 points · no flags
- Rejectedmlaurent · 2026-11-04 18:10:00 UTC“Compound precipitated at the top two concentrations” · v1 · auto-fit