AFN-002316-01 · EGFR WT
RejectedCRV-2026-00456-023 · v1 · auto-fitPlates PL-2026-0456, PL-2026-0457 (n = 2 replicates)4PL · Levenberg–Marquardt · unweighted, log₁₀[C]
Curve 9 / 10 in batch
Response vs concentration
- Rep. 1 · PL-0456
- Rep. 2 · PL-0457
- Masked
- IC50 95 % CI
Concentrations: 10-point, 1:3, 0.508 nM – 10.0 µMPoints used 20 / 20
Fit parameters — v1 · auto-fit
y = Bottom + (Top − Bottom) / (1 + 10^((log IC50 − x) · nH))| Parameter | Estimate | 95 % CI | Constraint |
|---|---|---|---|
| Top | 100.8 % | [97.8, 103.8] % | Free |
| Bottom | 4.8 % | [3.5, 6.1] % | Free |
| IC50 | 562 nM | [512, 617] nM | Free |
| pIC50 | 6.25 | ± 0.02 (SE) | Derived |
| Hill slope (nH) | 1.34 | [1.19, 1.48] | Free |
R² 0.998RMSE 1.6 %df 16Fit engine 1.2.0 · 20 / 20 points
Review decision
IC50
562 nM
95 % CI 512–617 nM · pIC50 6.25
Rejected: Signal drift across the plate, replicates disagree
History
append-only- v1 auto-fit createdsystem · 2026-11-04 17:05:40 UTCFit engine 1.2.0 · 20 / 20 points · no flags
- Rejectedmlaurent · 2026-11-04 18:10:00 UTC“Signal drift across the plate, replicates disagree” · v1 · auto-fit