■ Demo— not a validated GxP system — synthetic data

Affinis

PL-2026-0417 · EGFR L858R/T790M — TR-FRET kinase assay

QC passed

384-well · ASY-EGFR-TM-03 v2Read 2026-11-04 16:42:19 UTCInstrument EnVision-02 (mock import)Raw file sha256 6cbb…8681Imported by mlaurent

6,28549,687RFU · raw
HC
Cmpd A · 10-pt, 1:3 from 10 µM
Cmpd B · 10-pt, 1:3 from 10 µM
LC
123456789101112131415161718192021222324
ABCDEFGHIJKLMNOP

Masked — excluded from QC and fits, retained

Edge effect (HC, outer rows vs interior): +4.5 %

384 wells · 1 masked

Plate QC

rules QC-STD v3
Z′-factor0.86accept ≥ 0.50
Signal window (SW)
20.6≥ 2.0
Signal / background
6.82≥ 3.0
CV high control
3.5 %≤ 10 %
CV low control
3.8 %≤ 10 %
Masked wells
0.3 %≤ 5 %

Plates failing any rule are rejected automatically. Rejection can be overridden only by a signed exception.

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Audit trail — this plate
Timestamp (UTC)ActorEventDetail
2026-11-04 17:03:12 UTCsystemQC evaluated — passedQC-STD v3 · all rules met
2026-11-04 17:03:12 UTCsystemNormalization v2 computedExcludes D15 · v1 retained, read-only
2026-11-04 17:03:11 UTCmlaurentWell D15 masked“Dispense error — Dispense error — tip dispense error flagged in instrument log for D15”
2026-11-04 16:42:19 UTCsystemQC evaluated — passedQC-STD v3 · all rules met
2026-11-04 16:42:19 UTCsystemNormalization v1 computedμHC, μLC from cols 1–2 / 23–24 (n = 32 each)
2026-11-04 16:42:19 UTCsystemRaw data importedEnVision-02 export PL-2026-0417.csv · 384 wells · sha256 6cbb…8681 · stored write-once