PL-2026-0418 · EGFR L858R/T790M — TR-FRET kinase assay
Rejected384-well · ASY-EGFR-TM-03 v2Read 2026-11-04 16:42:19 UTCInstrument EnVision-02 (mock import)Raw file sha256 eb1c…5b0fImported by mlaurent
Automatic: QC-STD v3 not met
- PL-2026-0416Z′ 0.86Passed
- PL-2026-0417Z′ 0.86Passed
- PL-2026-0418Z′ 0.34Rejected
- PL-2026-0419Z′ 0.83Passed
- PL-2026-0420Z′ —Awaiting read
- PL-2026-0421Z′ —Awaiting read
5,12865,514RFU · raw
HC
Cmpd A · 10-pt, 1:3 from 10 µM
Cmpd B · 10-pt, 1:3 from 10 µM
LC
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Masked — excluded from QC and fits, retained
Edge effect (HC, outer rows vs interior): −0.1 %
384 wells · 0 masked
Plate QC
rules QC-STD v3Z′-factor0.34accept ≥ 0.50
- Signal window (SW)
- 1.7≥ 2.0
- Signal / background
- 6.83≥ 3.0
- CV high control
- 16.8 %≤ 10 %
- CV low control
- 13.6 %≤ 10 %
- Masked wells
- 0.0 %≤ 5 %
Plates failing any rule are rejected automatically. Rejection can be overridden only by a signed exception.
Well
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Audit trail — this plate
Open full audit trail| Timestamp (UTC) | Actor | Event | Detail |
|---|---|---|---|
| 2026-11-04 16:42:19 UTC | system | QC evaluated — rejected | QC-STD v3 · failed zPrime, signalWindow, cvHigh, cvLow · plate rejected automatically |
| 2026-11-04 16:42:19 UTC | system | Normalization v1 computed | μHC, μLC from cols 1–2 / 23–24 (n = 32 each) |
| 2026-11-04 16:42:19 UTC | system | Raw data imported | EnVision-02 export PL-2026-0418.csv · 384 wells · sha256 eb1c…5b0f · stored write-once |